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Thermo Fisher phosphate buffered saline dpbs thermo fisher scientific
Phosphate Buffered Saline Dpbs Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphate+buffered+saline+dpbs+thermo+fisher+scientific/PHOSPHATE+BUFFERED+SALINE+DPBS/pm41722571-242-174-178
Average 98 stars, based on 1 article reviews

phosphate buffered saline dpbs thermo fisher scientific - by Bioz Stars, 2026-10

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Clinical Proteomics:

Article Title: Multiple sclerosis severity variant in DYSF-ZNF638 locus associates with neuronal loss and inflammation
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Human brain tissue and CSF Netherlands Brain Bank https://www.brainbank.nl Antibodies and secondary reagents Rabbit-anti-human Olig2 – Alexa Fluor� 647 Abcam Cat# ab225100 RRID: - Donkey-anti-mouse IgG – Cy3 Jackson ImmunoResearch Cat# 715-165-150 RRID: AB_2340813 Donkey-anti-rabbit IgG – Cy3 Jackson ImmunoResearch Cat# 711-165-152 RRID: AB_2307443 FcR-Blocking Reagent Miltenyi Biotec Cat# 130-059-901 RRID: AB_2892112 Goat-anti-human SOX10 R&D Systems Cat# AF2864 RRID: AB_442208 Horse-anti-goat-IgG – biotin Vector Laboratories Cat# BA-9500 RRID: AB_2336123 Horse-anti-mouse-IgG – biotin Vector Laboratories Cat# BA-2000 RRID: AB_2313581 Horse-anti-rabbit-IgG – biotin Vector Laboratories Cat# BA-1100 RRID: AB_2336201 Mouse-anti-human APP A4 Sigma-Aldrich Cat# MAB348 RRID: AB_94882 Mouse-anti-human CD79A DAKO Cat# M705001-2 RRID: - Mouse-anti-human Dysferlin Leica Cat# HAMLET-CE RRID: - Mouse-anti-human NeuN Sigma-Aldrich Cat# MAB377 RRID: AB_2298772 Mouse-anti-human NeuN – Alexa Fluor�488 Sigma-Aldrich Cat# MAB377x RRID: AB_2149209 Mouse-anti-human neurofilament marker BioLegend Cat# 837901 RRID: - Rabbit-anti-human CD3 DAKO Cat# A045201 RRID: - Rabbit-anti-human ZNF638 Sigma-Aldrich Cat# HPA036784 RRID: AB_10672478 Streptavidin – Alexa Fluor�488 Jackson ImmunoResearch Cat# 016-540-084 RRID: AB_2337249 Chemicals, peptides, and recombinant proteins 3,3′-diaminobenzidine (DAB) DAKO Cat# K5007 Biotinylated tyramide Sigma-Aldrich Cat# SML2135 Bovine serum albumin (BSA) Sigma-Aldrich Cat# A7906 cOmplete ULTRA tablets, Protease Inhibitor Cocktail Merck Cat# 5892953001 Dulbecco’s phosphate-buffered saline (DPBS) Thermo Fisher Scientific Cat# 14190094 Dithiothreitol (DTT) Thermo Fisher Scientific Cat# R0861 Entallan Sigma-Aldrich Cat# 1.07961 Haematoxylin Merck Cat# 4305 Hoechst 33342 Thermo Fisher Scientific Cat# H3570 Hydrogen peroxide Merck Cat# 107209 MgCl2 Thermo Fisher Scientific Cat# AM9530G (Continued on next page) iScience 28, 112430, May 16, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mowiol Merkc Cat# 4705904 Sucrose Sigma-Aldrich Cat# 16104 Triton X-100 Sigma-Aldrich Cat# X-100 UltraPureTM 0.5M EDTA, pH8.0 Thermo Fisher Scientific Cat# 15575020 UltraPureTM 1M Tris-HCI, pH8.0 Thermo Fisher Scientific Cat# 15568025 Critical commercial assays DNeasy Blood & Tissue Kit Qiagen Cat# 69506 NF-light ELISA CE kit for CSF Uman Diagnostics Cat# 10-7001 RNEasy micro kit Qiagen Cat# 74004 SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian Library Prep Kit Takara Bio Cat# 634485 Vector Elite ABC kit Vector Laboratories Cat# PK-6100 RRID: AB_2336819 Deposited data Nuclei bulk RNAseq data This paper GEO: GSE273954 Tissue bulk RNAseq data Chen et al.13 GEO: GSE283092 Software and algorithms Application Suite X Leica N/A Bioconductor (v3.13) Huber et al.47 https://bioconductor.org/ biomaRt package (v.2.58.2) Durinck et al.48 Bioconductor dtangle (v.2.0.9) Hunt et al.49 https://cran.r-project.org/package=dtangle dupRadar (v1.12.1) Sayols et al.50 Bioconductor edgeR package (v3.34.1, 3.42.4) Robinson and Oshlack51 Bioconductor Emmeans package (v1.10.2) CRAN https://cran.r-project.org/package=emmeans fastp (v0.23.4), Chen et al.52 https://github.com/OpenGene/fastp FastQC (v0.11.9 & v0.12.1) Babraham Institute https://www.bioinformatics.babraham.ac.uk/ projects/fastqc/ featureCounts (v2.0.6) Liao et al.53 https://subread.sourceforge.net/ featureCounts.html FlowJo (v10.10.0) BD Biosciences https://www.flowjo.com/ gencode.v45.primary_assembly.annotation.gtf Gencode https://www.gencodegenes.org/human/ ggplot2 package (v3.5.1) Wickham54 https://cran.r-project.org/package=ggplot2 glmmTMB package CRAN https://CRAN.R-project.org/package=glmmTMB GRCh38.105 Ensembl https://www.ensembl.org/index.html GRCh38.primary_assembly.genome.fa (v.45) Gencode https://www.gencodegenes.org/human/ HISAT2 (v2.2.1) Kim et al.55 http://daehwankimlab.github.io/hisat2/ Homo_sapiens.GRCh38.105.gtf Ensembl https://www.ensembl.org/index.html HTseq (v1.99.2) Anders et al.56 https://htseq.readthedocs.io/en/master/ Fiji for ImageJ (v1.54f) Schindelin et al.57 https://imagej.net/software/fiji/ limma package (v3.56.2) Ritchie et al.58 Bioconductor lme4 package CRAN https://cran.r-project.org/package=lme4 PCAtools package (v2.14.0).

Marker:

Recombinant:

Article Title: Protocol for in vivo lineage tracing of regeneration-associated macrophages from injured skeletal muscle of adult mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-mouse CD209a (DC-SIGN; clone MMD3) (1:20) BioLegend Cat#833005; RRID: AB_2927969 APC anti-mouse NK-1.1 (clone PK136) (1:25) BioLegend Cat#108710; RRID: AB_313396 APC anti-mouse CD170 (Siglec-F; clone S17007L) (1:80) BioLegend Cat#155508; RRID: AB_2750236 APC-eFluor 780 anti-mouse CD11b (clone M1/70) (1:200) Invitrogen (eBioscience) Cat#47-0112-82; RRID: AB_1603193 Pacific Blue anti-mouse I-A/I-E (clone M5/114.15.2) (1:200) BioLegend Cat#107620; RRID: AB_493527 Brilliant Violet 711 anti-mouse Ly-6C (clone HK1.4) (1:40) BioLegend Cat#128037; RRID: AB_2562630 FITC anti-mouse Ly-6G (clone 1A8) (1:400) BioLegend Cat#127606; RRID: AB_1236488 PE anti-human/mouse CD109 (clone # 496920) (1:10) Bio-Techne/R&D Systems Cat#FAB4385P; RRID: AB_10643402 PE-Cyanine5 anti-mouse CD45.1 (clone A20) (1:500) Invitrogen (eBioscience) Cat#15-0453-82; RRID: AB_468759 Chemicals, peptides, and recombinant proteins Barium chloride Sigma-Aldrich Cat#202738-5G Dulbecco’s modified Eagle’s medium (DMEM) Corning Cat#10-013-CMR Penicillin and streptomycin Thermo Fisher Scientific (Gibco) Cat#15140122 Collagenase B Roche Cat#11088815001 Calcium chloride (CaCl 2 ) solution 2.5 M Jena Bioscience Cat#BU-103 RBC lysis buffer (10X) Santa Cruz Biotechnology Cat#sc-296258 UltraPure DNase/RNase-free distilled water Thermo Fisher Scientific (Invitrogen) Cat# 10977035 Fetal bovine serum (FBS) Sigma Cat#F9665 Horse serum (HS) Sigma Cat#H1138 Clodronate liposomes (5 mg mL − 1 ) LIPOSOMA SKU #CP-010-010 Isoflurane ISOVET 250 mL B. Braun Cat#469860 Sterile saline solution (NaCl 0.9%) 5 mL B. Braun Cat#3653800 Dulbecco’s phosphate-buffered saline (DPBS) Thermo Fisher Scientific (Gibco) Cat14190144 IC fixation buffer Thermo Fisher Scientific (Invitrogen) Cat#2865823 Trypan blue, 0.4% Thermo Fisher Scientific (Gibco) Cat#15250061 Experimental models: Organisms/strains Mouse: C57BL/6 (males and females) Charles River Strain #027; RRID:IMSR_CRL:027 Mouse: CD45.1 + /Ly5.1 (males and females) Charles River Strain #002014; RRID: IMSR_JAX:002014 Software and algorithms FlowJo v.10.10 BD Biosciences RRID:SCR_008520 GraphPad Prism 9 GraphPad Software RRID:SCR_002798 Other 70 μm cell strainers Corning Cat#431751 Centrifuge for 50 mL tubes Calservice Heratec,S.L. ..

Article Title: Modeling lipid homeostasis using stable isotope tracing and flux analysis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LKB1 antibody Cell Signaling Technologies Cat # 3047; RRID:AB_2198327 p-ACC S79 antibody Cell Signaling Technologies Cat # 3661; RRID:AB_330337 ACC antibody Cell Signaling Technologies Cat # 3662; RRID:AB_2219400 p-AMPKα T172 antibody Cell Signaling Technologies Cat # 2535; RRID:AB_331250 Actin antibody Sigma Aldrich Cat # A5441; RRID:AB_476744 anti-rabbit antibody Sigma Aldrich Cat # AP132P; RRID:AB_90264 anti-mouse antibody Millipore Sigma Cat # AP124P; RRID:AB_90456 Biological samples Mouse Lungs with tumors This Paper N/A Chemicals, peptides, and recombinant proteins [U-13 C 6 ] D-glucose Cambridge Isotope Laboratories CLM-1396 [U-13 C 3 ] L-serine Cambridge Isotope Laboratories CLM-1574 [U-13 C 2 ] L-glycine Cambridge Isotope Laboratories CLM-1017 D-glucose Sigma Aldrich G8270 L-serine Sigma Aldrich S4500 L-glycine Sigma Aldrich 1295800 DMEM Thermo Fisher Scientific Cat#11965-092 DMEM without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate Sigma Aldrich D5030 DMEM without glucose, amino acids, phenol red, sodium pyruvate, and sodium bicarbonate Cytiva Custom order RPM1640 Thermo Fisher Scientific 11875093 Fetal Bovine Serum (FBS) Thermo Fisher Scientific 16000-044 Penicillin-streptomycin Thermo Fisher Scientific 15130-122 Dubelcco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific 141190-144 Dimethyl sulfoxide Thermo Fisher Scientific BP231-100 Fumonisin B1 Enzo Life Sciences BML-SL220 Sodium Chloride Sigma Aldrich S9888 LiChrosolv 2-propanol Sigma Aldrich 1.02781 LiChrosolv methanol Sigma Aldrich 1.06035 LiChrosolv acetonitrile Sigma Aldrich 1.00029 Formic Acid Sigma Aldrich 5.43804 Water Sigma Aldrich 270733 Chloroform Sigma Aldrich 366927 Ammonium formate Sigma Aldrich 70221 Ammonium acetate Sigma Aldrich 73594 Methoxylamine hydrocloride Sigma Aldrich 89803 MSTFA Silylation Reagent Macherey-Nagel 701270.510 UltimateSPLASH One Mix Avanti Polar Lipids 330820 EquiSPLASH Avanti Polar Lipids 330731 SPB 18:0;O2 [d7] Avanti Polar Lipids 860658 SPB 18:1;O2 [D7] Avanti Polar Lipids 860657 glucosylceramide 18:1;O2[D7]/15:0 Avanti Polar Lipids 330729 lactosylceramide 18:1;O2[D7]/15:0 Avanti Polar Lipids 330727 (Continued on next page) Cell Metabolism 38, 1–14.e1–e6, March 3, 2026 e1 .. A549 and H1299 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) with 1%, 10%, or 20% FBS and 1% penicillin/streptomycin (P/S).

Article Title: Molecular basis for shifted receptor recognition by an encephalitic arbovirus.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-PCDH10 antibody Proteintech Cat#: 21859-1-AP; RRID: AB_2878929 Anti-VLDLR antibody GeneTex Cat#: GTX79552; RRID: AB_11171827 Anti-MXRA8 antibody MBL International Cat#: W040-3; RRID: AB_2801291 Rabbit IgG isotype Proteintech Cat#: 30000-0-AP; RRID: AB_2819035 Mouse IgG isotype BD Biosciences Cat#: BDB557351; RRID: AB_10050406 PE-conjugated donkey anti-rabbit F(ab’)2 fragment Jackson ImmunoResearch Cat#: 711-116-152; RRID: AB_2340599 PE-conjugated donkey anti-mouse F(ab’)2 fragment Jackson ImmunoResearch Cat#: 715-116-150; RRID: AB_2340798 APC-conjugated rat anti-DYKDDDDK (Flag) antibody BioLegend Cat#: 637307; RRID: AB_2561496 APC-conjugated rat isotype control antibody BioLegend Cat#: 402306; RRID: AB_3097097 Anti-Human IgG (Fc specific)–Peroxidase antibody Sigma Cat#: A0170; RRID: AB_257868 Anti-WEEV E2–E1 monoclonal antibody SKW11 Sutton et al.59 N/A Bacterial and virus strains Highlands J virus (strain B 230) ATCC Cat#: VR-622 Chemicals, peptides, and recombinant proteins DMEM Gibco Cat#: C11995500BT Expi293 Expression Medium Thermo Fisher Scientific Cat#: A1435101 Neurobasal medium Thermo Fisher Scientific Cat#: 21103 Glutamax Thermo Fisher Scientific Cat#: 35050 B-27 Thermo Fisher Scientific Cat#: 17504 Y-27632 Stemcell Technologies Cat#: 72302 Fetal bovine serum (FBS) ATLAS Biologicals Cat#: F-0500-D HEPES Thermo Fisher Scientific Cat#: 15630080 RPMI1640 Thermo Fisher Scientific Cat#: 11835055 Pen strep (penicillin-streptomycin) Gibco Cat#: 10378016 Opti-MEM Thermo Fisher Scientific Cat#: 31985088 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#: 14190-144 Pierce Chromogenic Endotoxin Quantification Kit Thermo Fisher Scientific Cat# A39552 MabSelectTM PrismA protein A affinity resin Cytiva Cat#: 17549801 Superdex 200 increase 10/300 GL column Cytiva Cat#: 28990944 Human PCDH10EC1-Fc Li et al. 15 N/A Human VLDLRLBD-Fc Clark et al. 34 N/A Human VLDLR LA(1–2)-Fc This study N/A Sparrow PCDH10EC1-Fc This study N/A Human MXRA8ect-Fc Clark et al. 34 N/A Human PCDH10EC1-twin-strep This study N/A Receptor associated protein (RAP) Clark et al.34 N/A Critical commercial assays ExpiFectamineTM 293 Transfection Kit Thermo Fisher Scientific Cat#: A14525 Lipofectamine 3000 Thermo Fisher Scientific Cat#: L3000150 e-Myco PCR detection kit Bulldog Bio Cat#: 25234 Deposited data WEEV CBA87 VLP density map This study EMD-47117 WEEV CBA87 VLP model This study PDB ID: 9DQX (Continued on next page) e1 Cell 188, 1–17.e1–e8, May 29, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER lentiGuide-Puro-garter snake PCDH10 Li et al.15 N/A lentiGuide-Puro-mouse PCDH10 Li et al.15 N/A lentiGuide-Puro horse PCDH10 Li et al.15 N/A WEEV CBA87 virus-like particle in pVRC plasmid Ko et al.32 N/A WEEV McMillan virus-like particle in pVRC plasmid Li et al.15 N/A WEEV Imperial 181 virus-like particle in pVRC plasmid This study N/A Western equine encephalitis virus (strain McMillan) molecular clone Gardner et al.60 N/A Software and algorithms RELION v3.1.4 Zivanov et al.61 N/A crYOLO v1.8.2 Wagner et al.62 N/A DeepEMhancer v20210511 Sanchez-Garcia et al.63 N/A MotionCor2 v1.6.4 Zheng et al.64 N/A CTFFIND-4.1 v4.1.14 Rohou and Grigorieff65 N/A UCSF ChimeraX v1.6.1 Pettersen et al.66 N/A PyMOL v3.0.2 https://www.pymol.org/pymol N/A Coot v0.9.8 Emsley et al.67 N/A Phenix v1.21rc1-5127 Adams et al.68 N/A AlphaFold2 Jumper et al.69 N/A GraphPad Prism v10.2.3 GraphPad N/A FlowJo v10.6.2 FlowJo, LLC N/A Incucyte S3 Software v2023B Sartorius N/A MEGA v11.0.10 MEGA Development Team N/A Other Quantifoil grids R 0.6/1 300 mesh, gold Electron Microscopy Sciences Cat#: Q350AR-06 Quantifoil grids R 2/2 300 mesh, gold Electron Microscopy Sciences Cat#: Q3100AR2 ll OPEN ACCESS Please cite this article in press as: Fan et al., Molecular basis for shifted receptor recognition by an encephalitic arbovirus, Cell (2025), https:// doi.org/10.1016/j.cell.2025.03.029 Article

Article Title: Mechanisms of HSV-1 helicase-primase inhibition and replication fork complex assembly.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains MAX Efficiency DH10BacTM cells Thermo Fisher Scientific Cat# 10361012 BL21 DE3 pLysS cells Novagen/Millipore Sigma Cat# 69451 Chemicals, peptides, and recombinant proteins Amylose Resin New England Biolabs Cat # E8021S cOmpleteTM, Mini, EDTA-free protease inhibitor cocktail Millipore Sigma Cat# 11836170001 Dithiothreitol (DTT) Sigma-Aldrich Cat# 10708984001 HSV-1 UL5 residues 1–882 with N-terminal MBP tag This paper N/A HSV-1 UL52 residues 1–1058 This paper N/A HSV-1 UL8 residues 1–750 with N-terminal MBP tag This paper N/A HSV-1 UL30 residues 43–1235 with an N-terminal polyhistidine tag This paper N/A HSV-1 UL42 residues 2–340 with N-terminal MBP tag This paper N/A HSV-1 UL5 residues 1–882 with N-terminal MBP tag with K356N substitution This paper N/A HSV-1 UL52 residues 1–1058 with F360R substitution This paper N/A HSV-1 UL52 residues 1–1058 with D628A substitution This paper N/A HSV-1 UL5 residues 1–882 with N-terminal MBP tag with F550A, Y648A, Y696A and N699A substitutions This paper N/A HRV-3C protease TaKaRa Cat# 7360 Nucleotide triphosphate (NTP) 100 mM each Thermo Fisher Scientific Cat# R0481 Superdex 200 increase Cytiva Cat# 28990944 Dulbecco’s phosphate-buffered saline (DPBS) Thermo Fisher Scientific Cat# 14190144 0.45 μm membrane (MCE Membrane Filter) Millipore Sigma Cat# HAWP14250 HiTrapTM Heparin HP affinity column Cytiva Cat# 45-000-057 tris (2-carboxyethyl) phosphine (TCEP) Oakwood Chemical Cat# M02624 HiTrap TALON® crude column Cytiva Cat # 28953809 Pritelivir Sigma-Aldrich Cat# SML3422 IM-250 Probe Chem Cat# PC-72690 Amenamevir MedChemExpress Cat# HY-14809 Deoxythymidine triphosphate (dTTP) Invitrogen Cat# 10219012 Bovine serum albumin (BSA) Sigma-Aldrich Cat# A7906 DNase I Thermo Fisher Scientific Cat# EN0521 RNAse T1 Thermo Fisher Scientific Cat# AM2283 RNAse A New England Biolabs Cat# T3018L (Continued on next page) ll OPEN ACCESS e1 Cell 189, 1–17.e1–e9, January 22, 2026 Please cite this article in press as: Yu et al., Mechanisms of HSV-1 helicase-primase inhibition and replication fork complex assembly, Cell (2026), https://doi.org/10.1016/j.cell.2025.11.041 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Cryo-EM map of HSV-1 replication fork complex This paper EMD-72764 Cryo-EM map of masked UL5 in replication fork complex This paper EMD-72754 Cryo-EM map of masked UL30 in replication fork complex This paper EMD-72755 Cryo-EM map of masked UL5–UL30 interface This paper EMD-72758 Cryo-EM map of HSV-1 replication fork complex (low resolution) This paper EMD-72757 Model of HSV-1 replication fork complex This paper PDB: 9YC9 Cryo-EM map of HSV-1 H/P complex bound to pritelivir This paper EMD-72786 Cryo-EM map of masked UL5-UL52 in pritelivir-bound H/P This paper EMD-72784 Cryo-EM map of masked UL52-UL8 in pritelivir-bound H/P This paper EMD-72785 Model of HSV-1 H/P complex bound to pritelivir This paper PDB: 9YCT Cryo-EM map of HSV-1 H/P complex bound to IM-250 This paper EMD-72781 Cryo-EM map of masked UL5-UL52 in IM-250-bound H/P This paper EMD-72780 Model of HSV-1 H/P complex bound to IM-250 This paper PDB: 9YCP Cryo-EM map of HSV-1 H/P complex bound to amenamevir This paper EMD-72789 Cryo-EM map of masked UL5-UL52 in amenamevir-bound H/P This paper EMD-72787 Cryo-EM map of masked UL52-UL8 in amenamevir -bound H/P This paper EMD-72788 Model of HSV-1 H/P complex bound to amenamevir This paper PDB: 9YCV Experimental models: Cell lines Sf9 cells (Spodoptera frugiperda) ThermoFisher Cat# 11496015, RRID: CVCL_JF76 Expi293FTM cells Thermo Fisher Scientific Cat#: A14527; RRID: CVCL_D615 Oligonucleotides Forked DNA template: 5 ′ -TCC CGC CCG

Protease Inhibitor:

Article Title: Prioritization and functional validation of target genes from single-cell transcriptomics studies
Article Snippet: Anti-α-Tubulin; clone: DM1A (dilution: 1:1000) Sigma-Aldrich Cat#T6199 Anti-CCDC85B Antibody; polyclonal (dilution: 1:500) Proteintech Cat#18282-1-AP Anti-CD31 Antibody (FITC); clone: 390 (dilution: 1:100) Thermo Fisher Scientific Cat#11-0311-82 RRID: AB_465012 Anti-CD45 Antibody (PE-Cy7); clone: 30-F11 (dilution: 1:500) Thermo Fisher Scientific Cat#25-0451-82 RRID: AB_2734986 Anti-Connexin 43 Antibody (GJA1); clone: F7 (dilution: 1:500) Santa Cruz Biotechnology Cat#SC-271837 Anti-CD93 Antibody; clone: R139 (dilution: 1:500) Thermo Fisher Scientific Cat#14-0939-82 Anti-ICAM-2 Antibody (CD102), Alexa Fluor 647; clone: 3C4 (dilution: 1:50) Thermo Fisher Scientific Cat#A15452 RRID: AB_2534465 Anti-ELTD1 Antibody; clone: CL4164 (dilution: 1:500) Thermo Fisher Scientific Cat#MA5-24705 Anti-GAPDH; clone: 14C10 (dilution: 1:1000) Cell Signaling Cat#2118 Anti-MYH9 Antibody; polyclonal (dilution: 1:500) Thermo Fisher Scientific Cat#PA5-17025 Anti-TCF4 Antibody; clone: D-4 (dilution: 1:500) Santa Cruz Biotechnology Cat#SC-166699 .. [3H]-thymidine Perkin Elmer Cat#NET355L005MC ACK Lysing Buffer Gibco Cat#A10491-01 Antibiotic-antimycotic Thermo Fisher Scientific Cat#15240062 Bovine serum albumin (BSA Fraction V) Sigma-Aldrich Cat#10735086001 Collagenase type I Thermo Fisher Scientific Cat#17018029 Dispase Thermo Fisher Scientific Cat#17105-041 DMEM, high glucose Thermo Fisher Scientific Cat#11965092 DNase I Sigma-Aldrich Cat#D4527-10KU ECGS (endothelial cell medium growth supplement mix) PromoCell Cat#C-39216 EDTA Sigma-Aldrich Cat#ED2P-500G EGM2 (Endothelial growth medium) PromoCell Cat#C-22011 Endothelial cell growth factor supplements (ECGS/Heparin) PromoCell Cat#C-30120 Fetal bovine serum (FBS) Thermo Fisher Scientific Cat#16000044 FITC-dextran Sigma-Aldrich Cat#FD2000S Fixable Viability Dye eFluorTM 780 Thermo Fisher Scientific Cat#65-0865-14 Gelatin from bovine skin Sigma-Aldrich Cat#G9391 Glutamine Thermo Fisher Scientific Cat#25030149 Glycerol Merck Millipore Cat#1.04091.1000 Hoechst 33258 Sigma-Aldrich Cat#B2261 Ketamine 100 mg/mL Eurovet Animal Health B.V. NIMATEK KnockOutTM DMEM Thermo Fisher Scientific Cat#10829018 Medium 199, HEPES Thermo Fisher Scientific Cat#22340020 MEM NEAA Thermo Fisher Scientific Cat#11140035 Mitomycin C Sigma-Aldrich Cat#M0503-2MG Methylcellulose 4000 cP Sigma-Aldrich Cat#M0512 Opti-MEM Thermo Fisher Scientific Cat#11058021 Paraformaldehyde Merck Millipore Cat#8.18715.1000 Penicillin/streptomycin Thermo Fisher Scientific Cat#15140122 Phosphate buffered saline (DPBS) Thermo Fisher Scientific Cat14190094 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat#89901 Phosphatase inhibitors (PhosSTOP) Roche Cat# 04906837001 ProLong Gold antifade reagent Thermo Fisher Scientific Cat#P36934; Protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail) Roche Cat#11873580001 Sodium dodecyl sulphate Acros Organics Cat#230425000 Sodium pyruvate Thermo Fisher Scientific Cat#11360070 SuperSignal West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific Cat#34095 TaqMan Fast Universal PCR Master Mix (2X) Thermo Fisher Scientific Cat#4364103 TCA (trichloroacetic acid) Sigma-Aldrich Cat#T6399 Trypsin-EDTA (0.25%) Thermo Fisher Scientific Cat# 25200056 Xylazine VMD Cat# XYL-M 2% .. CYTO-ID® Green long-term cell tracer kit Enzo Cat#ENZ-51036-K025 CYTO-ID® Red long-term cell tracer kit Enzo Cat#ENZ-51037-K025 in vivo-jetPEI® 0.5 mL Polyplus Cat#101000030 Lipofectamine RNAiMAX Thermo Fisher Scientific Cat#13778150 Pierce ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 PureLink RNA Mini Kit Thermo Fisher Scientific Cat#12183018A RNeasy Micro Kit QIAGEN Cat#74004 Script cDNA synthesis kit Bio-Rad Cat#1708891 SuperScript III First Strand cDNA synthesis kit Thermo Fisher Scientific Cat#18080051

Saline:

Modification:

Red Blood Cell Lysis:

Liposomes:

Sterility:

other:

Article Title: Chronic stress increases metastasis via neutrophil-mediated changes to the microenvironment.
Article Snippet: In brief Chronic stress is linked to increased metastasis in cancer patients, but the underlying mechanisms remain unclear.. In this study, He et al. show that chronic stress increases metastasis by affecting neutrophils.. Chronic stress, via glucocorticoids, alters neutrophils’ circadian rhythm and establishes a metastasis-promoting microenvironment by inducing neutrophil extracellular trap formation.

Article Title: Nouveau Benzo-Mimetics of 17R-Resolvin D2 are Potent Resolution Agonists for Inflammation
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies APC anti-human CD206 BD Pharmingen Cat# 550889; clone 19.2; RRID: AB_398476 PE anti-human CD163 Biolegend Cat# 333606, clone GH1/61; RRID: AB_1134002 APC/Cy7 anti-mouse CD45 BD Cat#557659; RRID: AB_396774 BV421 anti-mouse Ly6C Biolegend Cat#128032; RRID: AB_256178 AF700 anti-mouse Ly6G Biolegend Cat#127622; RRID: AB_10643269 PE/Cy7 anti-mouse F4/80 Biolegend Cat#123114; RRID: AB_893478 Bacterial and virus strains Escherichia coli serotype O6:K2:H1 NCBI NCBI Taxonomy ID 2990471 Biological samples Deidentified leukopack Boston Children’s Hospital Blood Bank NA Chemicals, peptides, and recombinant proteins Ficoll-Histopaque-1077 density-gradient Sigma Cat#10771-500ml RPMI 1640 ThermoFisher Scientific Cat#21870092 Fetal calf serum ThermoFisher Scientific Cat#16000-044 L-glutamine Fisher Cat#BW-17-605E Penicillin-streptomycin ThermoFisher Scientific Cat#15140122 Recombinant human macrophage colonystimulating factor (hr-MCSF) PeproTech Cat#300-25 Recombinant human interleukine-4 (IL-4) PeproTech Cat#200-04 Recombinant human granulocyte colonystimulating factor (hr-GMCSF) PeproTech Cat#300-03 Resolvin D2 Cayman Chemical Cat#10007279 Paraformaldehyde 16% Electron Microscopy Cat#15710-S Human TruStain FcXTM Biolegend Cat#422302 Methanol, Optima® LC/MS Grade Thermo Fisher Scientific Cat#A456-4 Water, Optima® LC/MS Grade Thermo Fisher Scientific Cat#W6-4 Formic acid, ACS reagent, ≥96% Sigma-Aldrich Cat#695076 D5-RvD2 Cayman Chemical Cat#11184 D5-RvD3 Cayman Chemical Cat#19512 D5-LXA4 Cayman Chemical Cat#10007737 D5-MaR1 Cayman Chemical Cat#21823 D4-LTB4 Cayman Chemical Cat#320110 D8-15S-HETE Cayman Chemical Cat#334720 D8-5S-HETE Cayman Chemical Cat#334230 NaBH4 Sigma Aldrich Cat#21346-2 Isoflurane Covetrus Cat#11695067772 Zymosan A from Saccharomyces cerevisiae Sigma-Aldrich Cat#Z4250 Luria-Bertani broth base Invitrogen Cat#12780-052 15-hydroxy Prostaglandin Dehydrogenase D50H variant (human, recombinant) Cayman Chemical Cat#34486 NAD+ (free acid) Cayman Chemical Cat#16077 BacLight Green Molecular Probes Cat#B35000 Dulbecco’s Phosphate Buffered Saline (DPBS) Thermo Fisher Scientific Cat#14040-133 (Continued on next page) e1 iScience 28, 114201, December 19, 2025

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Protease Inhibitor:

Saline:

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Red Blood Cell Lysis:

Liposomes:

Sterility:

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